# Biocides and Log Kill Explained

### All our biocides, algaecides and fungicides are 100% plant-derived, we do not use synthetic or petrochemical ingredients, all our products are certified and tested under the Biocidal Product Regulations (BPR) EU 528/2012 and tested to BS EN standards. They are biodegradable and safe for humans, plants and animals.

### What is Log Kill and why does it matter?

Disinfection professionals today are generally concerned with what percentage of a given germ is killed by a particular process or disinfectant. The highest percentage that is generally used is 99.9999%. In scientific research papers, this percentage is written as “ a 6 log10 reduction”, but in medical shorthand, it’s known as “a greater-than 6-log reduction” or “a log-6 kill rate.” ‘Log’ is short for logarithm, a mathematical term for a power to which a number can be raised.

Log rates are calculated using a logarithmic scale. Log reduction stands for a 10-fold (or one decimal point) reduction in bacteria, meaning the disinfectant or biocide reduces the number of live bacteria by 90 per cent for every step.

The log reduction achieved by a decontamination process is a measure of the reduction in the concentration of a contaminant and therefore the reduction of risk.

The name reflects that the measure is expressed on a logarithmic scale, using base 10. An increase by 1 corresponds to a reduction in the concentration of the contaminant by a factor of 10. A 0-log reduction means that there is no reduction.

A 1-log reduction would correspond to a reduction of 90 per cent from the original concentration. A 2-log reduction corresponds to a reduction of 99 per cent from the original concentration and so on.

Mathematically, the log reduction is the base-10 logarithm of the ratio between the concentrations before and after treatment.

Generally, you can say that log Kill is a description of how effective a product is at killing pathogens and Log Reduction is the description of how much of the pathogen will be killed.

To help understand the value of each additional “log”, let’s do the sums for a small colony of a million MRSA bacteria on a work surface in a hospital in this instance we are using the 'Log Kill as used to describe the efficacy of our Biocidal products:

• A 1-log kill reduces the colony to 100,000 MRSA bacteria after a 90% reduction;

• A 2-log kill reduces the colony to 10,000 bacteria after a 99% reduction

• A 3-log kill reduces the colony to 1,000 bacteria after a 99.9% reduction;

• A 4-log kill reduces the colony to 100 bacteria after a 99.99% reduction;

• A 5-log kill reduces the colony to 10 bacteria after a 99.999% reduction;

• **A 6-log kill reduces the colony to 1 MRSA bacterium after a 99.9999% reduction.**

In terms of infection control, ‘Log Reductions’ convey how effective a product is at reducing pathogens. The greater the log reduction the more effective the product is at killing bacteria and other pathogens that can cause infections.

During product efficacy testing, the microbiology laboratories count the number of colony forming units (CFU's is shorthand for the actual number of individual bacteria present) of the given pathogen at the start of the test. They then apply the disinfection product being tested, alongside a control product and wait the required test time before recounting the number of CFU's present.

The result of the difference between the control and the test product is then expressed as a Log reduction. For example, if the number of CFU's in the control was found to be 1,000,000 (or 10^{6}) and the end result using the product was only 1,000 (10^{3}), that would be a Log reduction of 3 or a reduction of 99.9% as in the illustration below.

As a basic rule of thumb, for every additional Log reduction number, you add a 9 to the percentage reduction – so a log reduction of 3, as illustrated above, is a 99.9% reduction compared with a log reduction of 6 which is equivalent to a 99.9999% reduction.

Log 6 Kill is regarded as a medical grade sanitising and disinfecting agent.

### Pathogens killed by the active ingredient in our biocides:

**Bacteria:**

Campylobacter jejuni

Dipiodia natalensis

Escherichia coli

Geotrichia coli

Klebsiella pentoaceticus

Legionella pneumophila (NCTC 11192)

Listeria monocytogenes

MRSA (clinical strain)

Mycobacterium fortutium (NCTC 8573)

Proteus vulgaris

Pseudomonas aeruginosa (ATCC 15442)

Salmonella cholerasuis

Salmonella typhimurium (DT004)

Staphylococcus aureus (NCTC 6571)

Staphylococcus pyogenes

Staphylococcus sp.

Streptococcus faecalis

Clostridium difficile

Trichophyton mentagrophytes

**Yeast and Fungi:**

Aspergillus flavus

Aspergillus niger

Aspergillus terreus

Botrutis cinerea

Candida albicans

Candida glabrata

Chaetonium globosum

Cladosporium

Collectotricum sp.

Fusarium sp.

Geotrichum candidium

Mucor sp.

Penicillium sp.

Penicillium digitatum

Penicillium funiculosum

Penicillium italicum

Penicillium roqueforti

Phomopsis ortl

Pullularia pullulans

Pythium sp.

Trichophyton interdigitale

**Viruses:**

Human Rhinovirus – Retroscreen Virology

Influenza A – Retroscreen Virology

Human Immunodeficiency Virus (HIV)

African swine fever

Avian influenza

Foot & mouth disease

Gumboro virus

Herpes virus type 1 & type 2

Herpes zoster

Hepatitis A & B

Newcastle disease

Severe Acute Respiratory Syndromes (SARS)

**Protozoa:**

Histomonas meleagradis

Giardia lamblia

Entamoeaba histolytica

Blastocystis hominis